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chef mapper xa system  (Bio-Rad)


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    Structured Review

    Bio-Rad chef mapper xa system
    Chef Mapper Xa System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 726 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chef+mapper+xa/CHEF+Mapper+XA+Chiller+System/pmc13122175-115-23-27
    Average 96 stars, based on 726 article reviews
    chef mapper xa system - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Pulsed-Field Gel:

    Article Title: Comprehensive genome annotation of Trilocha varians , a new model species of Lepidopteran insects
    Article Snippet: .. The digested fragments were fractionated and collected using CHEF Mapper XA pulsed-field gel electrophoresis system (Bio-Rad). .. The extracted DNA fragments were ligated to the pBeloBAC11 vector, and the ligates were transformed by electroporation (GenePulser II, Bio-Rad) into DH5α Electro-Cells (TaKaRa).

    Article Title: Strategic targeting of Cas9 nickase expands tandem gene arrays
    Article Snippet: DNA digested with or without EcoRI was subjected to 1% and 0.8% Certified Megabase Agarose (Bio-Rad) in 0.5× TBE and 1× TAE, respectively. .. Pulsed-field gel electrophoresis (PFGE) was performed using CHEF mapper XA (BioRad) according to the manufacturer’s instructions. ..

    Article Title: Adaptive Radioresistance of Enterohemorrhagic Escherichia coli O157:H7 Results in Genomic Loss of Shiga Toxin-Encoding Prophages.
    Article Snippet: Phage DNA was extracted from O/N cultures of E. coli K-12 MG1655-U using the ZymoPURE Miniprep kit (Cedarlane, Burlington, ON, Canada). .. Pulsed-field gel electrophoresis (PFGE) was performed with a CHEF Mapper XA from Bio-Rad with the following parameters: separation from 5 kb to 500 kb, calibration factor of 1.0, buffer of 0.5 Tris-borate-EDTA (TBE), temperature of 14°C, 1.0% pulsed field certified (PFC) agarose, gradient of 6.0 V/cm, run time of 15 h 16 min, included angle of 120°, initial switch time of 0.22 s, final switch time of 8.53 s, and linear ramping factor. ..

    Article Title: Strategic targeting of Cas9 nickase expands tandem gene arrays
    Article Snippet: DNA digested with or without EcoRI was subjected to 1% and 0.8% Certified Megabase Agarose (Bio-Rad) in 0.5× TBE and 1× TAE, respectively. .. Pulsed-field gel electrophoresis (PFGE) was performed using CHEF mapper XA (BioRad) according to the manufacturer’s instructions. ..

    Incubation:

    Article Title: Prevalence, toxin-genotype distribution, and transmission of Clostridium perfringens from the breeding and milking process of dairy farms.
    Article Snippet: This study aimed to elucidate the distribution, transmission, and cross-contamination of Clostridium perfringens during the breeding and milking process from dairy farms.. The prevalence of 22.3% (301/1351) yielded 494 C. perfringens isolates; all isolates were type A, except for one type D, and 69.8% (345/494) of the isolates carried atyp. cpb2 and only 0.6% (3/494) of the isolates carried cons. cpb2.. C. perfringens detected throughout the whole process but without type F. 150 isolates were classified into 94 pulsed-field gel electrophoresis (PFGE) genotypes; among them, six clusters contained 34 PFGE genotypes with 58.0% isolates which revealed epidemic correlation and genetic diversity; four PFGE genotypes (PT57, PT9, PT61, and PT8) were the predominant genotypes.

    Marker:

    Article Title: Prevalence, toxin-genotype distribution, and transmission of Clostridium perfringens from the breeding and milking process of dairy farms.
    Article Snippet: This study aimed to elucidate the distribution, transmission, and cross-contamination of Clostridium perfringens during the breeding and milking process from dairy farms.. The prevalence of 22.3% (301/1351) yielded 494 C. perfringens isolates; all isolates were type A, except for one type D, and 69.8% (345/494) of the isolates carried atyp. cpb2 and only 0.6% (3/494) of the isolates carried cons. cpb2.. C. perfringens detected throughout the whole process but without type F. 150 isolates were classified into 94 pulsed-field gel electrophoresis (PFGE) genotypes; among them, six clusters contained 34 PFGE genotypes with 58.0% isolates which revealed epidemic correlation and genetic diversity; four PFGE genotypes (PT57, PT9, PT61, and PT8) were the predominant genotypes.

    Agarose Gel Electrophoresis:

    Article Title: Back to the roots, desiccation and radiation resistances are ancestral characters in bdelloid rotifers
    Article Snippet: .. Plugs were loaded in a 0.8% agarose gel (Lonza, Rockland, ME, USA), and PFGE was performed on a BIORAD CHEF Mapper XA according to the following parameters: migration time = 22 h, temperature = 14 °C, volts/cm = 5.5, switch angle = 120°, switch times = 60 to 185 s with a linear ramp (resolution ranging from 225 to 1600 kb). ..

    Migration:

    Article Title: Back to the roots, desiccation and radiation resistances are ancestral characters in bdelloid rotifers
    Article Snippet: .. Plugs were loaded in a 0.8% agarose gel (Lonza, Rockland, ME, USA), and PFGE was performed on a BIORAD CHEF Mapper XA according to the following parameters: migration time = 22 h, temperature = 14 °C, volts/cm = 5.5, switch angle = 120°, switch times = 60 to 185 s with a linear ramp (resolution ranging from 225 to 1600 kb). ..



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